Happy Holidays Mushroom people 🤘☮️🤘

We all starting somewhere. There's my first home lab set up. Im about to buy a flowhood soon and epoxy paint the desk, now i'm using a homemade glovebox. I got everything I need to make it work.

What the Cordy-Cult doesn't want you to know about Cordyceps Militaris Preservation: Peep this very interesting 2024 study that was passed along to me a week or so ago. In essence if you are preserving known mating pairs of CM in their monokaryotic forms (through Deep Freezing/LN/Freeze Drying) and combine once your ready to expand to a round of LC and then fruiting substrate you will never experience "senescence"...as this study points out, our perception of "senescence" might be one thing but in fact in the case of C. Militaris it looks like a dominant monokaryon kicks the other Mon out after a series of expansions essentially rendering a dikaryon, monokaryotic once again. 

https://drive.google.com/file/d/1gxqBeIbpvyYTtMGPyu3yiw2vimbfPIfd/view?usp=sharing

A deep dive that follows along this specific protocol is in the works to see if it can be replicated! Stay stoked and share your fungal-finds homies!

-Erik

"Hi everyone!

I’d like to hear your opinions and experiences: Is it better to use more or less nutrients in liquid culture and agar? How can this affect the subsequent growth of mycelium and fruiting bodies? What are the advantages and disadvantages of using higher or lower nutrient concentrations in these media? Thanks in advance for your responses!"

Great job on the dehydration deep dive! You have a lot of great info on there in regard to in vitro fruiting. 

What are you guys finding to be an ideal climate in the grow space? I imagine the humidity can be greatly reduced in contrast to fruiting outside of the bag. Are you prioritizing FAE in the space? 

How's the space mission grains doing? I've eaten mushrooms and went to space for sure/Np but never eaten mushrooms "from" space. 

What were the conditions for the mycelium in space?  Was this the same space trip sporeworks stuff was on?  

Entering back into King Oyster production after taking the summer off. Usually our issues arise during fruiting, but the first two batches we have noc’d are getting some bacterial contamination during incubation. Anyone else noticing this? Think this might be an issue during shaking? I’m noticing the bottoms aren’t colonizing quite as quickly then the grossness manifests up top. Possibly over hydration too, but I know our first batch was at 60% on the dot after testing.

Thanks so much for the content Mycowizards! 

I have a hypsozigus tessalatus-beech (spelling butchered) culture I cloned from store bought I'm thinking of growing once or twice before I buy a plate from you guys but I know almost nothing about it. On grain it grows really slowly but really dense. What kind of incubation time am I looking at? Is it fast fruiting  like oysters or need to sit a long time like chestnut or shiitake? Also just got brick caps to fruit the first time recently but really disappointed. Only 6 mushrooms and I can't even find anyone that has got them to fruit before, that was like 5 months of incubation on the successful block....... Not successful but it fruited. Cased with non sterile compost, stamets book noted they need micro organisms in the casing to initiate a pin set. Any idea how to grow them properly? If not no worries it's just a side project 

Workman interview.  

Any essential equipment for tincture production that has made your process easier/more efficient? We have some pretty basic alcohol extraction and boiling extraction methods. I have seen some people talk about industrial strength grinders as a way to increase surface area and extraction.