What the Cordy-Cult doesn't want you to know about Cordyceps Militaris Preservation: Peep this very interesting 2024 study that was passed along to me a week or so ago. In essence if you are preserving known mating pairs of CM in their monokaryotic forms (through Deep Freezing/LN/Freeze Drying) and combine once your ready to expand to a round of LC and then fruiting substrate you will never experience "senescence"...as this study points out, our perception of "senescence" might be one thing but in fact in the case of C. Militaris it looks like a dominant monokaryon kicks the other Mon out after a series of expansions essentially rendering a dikaryon, monokaryotic once again.
https://drive.google.com/file/d/1gxqBeIbpvyYTtMGPyu3yiw2vimbfPIfd/view?usp=sharing
A deep dive that follows along this specific protocol is in the works to see if it can be replicated! Stay stoked and share your fungal-finds homies!
-Erik