Hello ya'll! There has been a lot of talk as of late in different forums and groups about the efficacy and practical use of old study protocols specific to chemical dedikaryotization, so i thought i would share this interesting and easy protocol for isolating two monokaryons from a single dikaryon. I'm not sure how many folks are getting as into the weeds as we are about mating and DNA analysis but this is a pretty cool primer for what is possible. Some practical reasons for needing to preform dedikaryotization is if you are trying to generate a new phenotype from a dikaryon that cannot produce mating pairs within its own spore production (i.e. Shimofuri Hiratake or PE or Enigma, among many others in the gourmet, active and medicinal world). The process of dedikaryotization allows you to pull the mon parents of literally ANY Di and from there perform crosses to continue breeding. Anyways, I hope this is interesting and insightful for ya'll and hopefully in the next couple months we will put together a full deep dive on the matter!
Fuck Yeah CODeuce control 🤌. Now I can play that moving on up song and be soulful as I move the blocks into the newish tent. 🤘
Yay I got up early enough to case these King Oysters 🤘☮️🤘. If I read Gregor says to case the Kings.....bam. Cased wicked hard .
Hey everyone, are there any videos that delve a bit deeper into Stroma? I occasionally encounter difficulties with these dense mycelium blobs, and I have no clue about their origin. 🙃 Any help or insights would be greatly appreciated!"
Would anyone like to take a shot as to what is causing this weird growth on my oyster spawn? I've gotten this kind of growth before and the spawn wasn't contaminated ? Have you seen this before? (The darker brown looking dust is the gypsum). Then second half pictures are from a different lot.
We have an FFU that was installed in a ceiling with its back exposed to an attic, where it was used for a couple years and then not used for 7 years. Is it salvageable?
I was thinking about changing the HEPA filter on it (in addition to changing the pre-filter of course), but it doesn't seem like the FFU is made to be taken not made to be taken apart easily.
What is y'alls' protocol for FFU maintenance and for changing or not the filters?
Do you ever perform a test of the HEPA for leaks?
As always, thanks a ton for the help!
Hello fellow heathens!
I'm curious about proportional injectors for peroxide in my misting system. I currently use a 55 gal drum reservoir that feeds into a HART setup. I have been using bleach when filling the drum from an outdoor hydrant to keep the water from getting too funky. I'm curious about using peroxide as an alternative treatment.
What product/injectors are people using to inject into misting lines? I'm familiar with dosatron style fertilizer injectors but they require higher flow rates. I know I can just add peroxide to my large reservoir but I'm curious if anyone is injecting the peroxide into the misting line as it is humidifying. I've heard mention of peristaltic pumps but I don't quite understand the specifics around them.
Thanks!
Hi everyone!
I was wondering if you had any tips about placement of the CO2 meter (yes, I listened to your advice and got one finally!) I'm experimenting with different positions, so far chest level at the back of the greenhouse seems to work well but maybe you have some pro tips that could be useful!
Thanks for all the content, it's so dope.