Hey George, so after endless amounts of transfers in a day sterilizing tweezers or a scalpel in the red hot ceramic sterilizer, agar goop gets all gummed up on the transfer utensils, so after each transfer we dunk our tools in alcohol to get the agar goop off the utensils before putting them into the ceramic sterilizer again. This way we don't bake sticky, crusty agar goop onto the transfer tools, because that would be a contamination vector without a doubt. Also having done that dance without the alcohol when you cool the transfer tools in the agar and it is covered is blacked agar goop, it splatters all over the top of the agar dish causing a gnarly splattering of black debris. Maybe we should do a Lab protocol video?! bring back some old school Roger Rabbit style goodness to the channel?!
Erik Lomen
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30 Oct 12:18
I feel this for sure, it is largely due to over humidification in a fruiting room without proper air ventilation. I've seen it with numerous species and the fix for most folks is a redesign using something like a Hart Nozzle. Those lions soak up a shit ton of water and need a lot of evaporation in air exchanges to keep them form bruising, that's for sure!