Gregor Awesome! Thanks for that. So far, pretty intuitive and logical stuff. I tried folding the bag over after hearing about that on your deep dive video and it was still drying out, although that doesn't mean it didn't decrease the drying, I just didn't observe it carefully enough. The specifics parameters that you have tried and tested are very helpful. When the literature states the mycelium tolerates a certain ppm, (over 5kppm), I'm assuming that it is inside the bag so you want to keep it significantly lower in the room, as you do.

So far I captured 1 erinaceus, 1 coralloides and 1 americanus (I believe). Got them on spawn now and excited to fruit my first wild Hericiums. Any tips on substrate, etc. are greatly appreciated.

So far I captured 1 erinaceus, 1 coralloides and 1 americanus (I believe). Got them on spawn now and excited to fruit my first wild Hericiums. Any tips on substrate, etc. are greatly appreciated.

BenJamin Harderhmm my concern with this one is that you can't position the bag in a way that it is still receiving HEPA air up to the point of sealing. In other words, the sealer is going to block laminar flow clean air and potentially cause dirty air to get in the bag. I guess if you hold the bag completely shut as you stick it in the sealer it could work. I've never used one of these style sealers

Phillipe Kenny I'm guessing the heat accelerated the metabolic rate of the oysters, especially as they became thick and that cause that spike in CO2

Thanks for such a timely and complete response and orientation. Once you open the bag and break up the "tumor" growth, it has a distinct smell as well, right?Another factor which I updated on my post is that the CO2 level increases room 500ppm to 1300 ppm, not sure for how many days because I wasn't tracking. I wonder if that was a major cause? 

Interesting thoughts on thickness of sub and using the bag without a tray 

That's cool to hear you are doing water preservation of your gourmet cultures. I haven't seen many people who have tried it. I've been doing it for a while but haven't got enough data on viability and vigor. especially as related to yield. Look forward to hearing your results and hopefully sharing results too