BenJamin HarderActually no, it was a long cut and then two layers of micropore tape closed the cut to create a filter for it to fruit though...tip from Lenny SR. Helps to keep the sub from greening out.Yeah they do not seem to require any conditioning unless you live someplace extreme. 

What is your elevation ? Might be why you are struggling to get 250f....

Can you link to the probe?

Thanks,

So essentially you can use this to generate bank of monokaryons of whatever strain/species you're working with but without needing to germinate spores or even needing spores at all, correct? And then from there you could use those monokaryons in breeding trials like if you wanted to say cross snow with BPK ect?

Gregor Interesting! So you guys are not sending LC to rice sub but rather rice/oat grain spawn (from agar wedges) to bulk sub?

GregorThanks for the reply, I probably should have specified I was also asking in terms saprobic fungi not just ascomycetes when I was talking about linear growth total VS instances of transfer. Do you think the distinction would more applicable to saprobic or not so much?Thanks!

Gregor Interesting...so are you quantifying the "age" and subsequent senescence based on the linear measurement for total growth of that culture line from spore to notable degradation OR are you simply counting transfers themselves regardless of the amount of growth that line actually ran? There is a huge difference it seems between those samples. In one case the specific line simply keeps on growing without hitting that "wall" as frequently. So then a larger plate (more room) or larger quantity of LC perhaps combined with colder growing temps to slow things down would seem appropriate if you wanted to reduce those degradations issues. Total length measured in mm would be higher.But then if the total lifespan length of running for that line is paramount then transfers themselves should not be the issue ? Like you could frequently transfer before it runs out of food or other obstacle that might shift the metabolism. You would then just be keep the total mm length of running to a minimum.