Erik Lomen

Posted

13 Dec 20:55

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Hello ya'll! There has been a lot of talk as of late in different forums and groups about the efficacy and practical use of old study protocols specific to chemical dedikaryotization, so i thought i would share this interesting and easy protocol for isolating two monokaryons from a single dikaryon. I'm not sure how many folks are getting as into the weeds as we are about mating and DNA analysis but this is a pretty cool primer for what is possible. Some practical reasons for needing to preform dedikaryotization is if you are trying to generate a new phenotype from a dikaryon that cannot produce mating pairs within its own spore production (i.e. Shimofuri Hiratake or PE or Enigma, among many others in the gourmet, active and medicinal world). The process of dedikaryotization allows you to pull the mon parents of literally ANY Di and from there perform crosses to continue breeding. Anyways, I hope this is interesting and insightful for ya'll and hopefully in the next couple months we will put together a full deep dive on the matter! 

3

Hell Yea!!!!

28 Nov 12:35

Slick! We just recorded a Deep Dive for ya on Kings! Coming soon!!!

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27 Nov 12:49

We are getting increasingly stoked on DNA sequencing and I have a real interested in sequencing some stroma along side some healthy mycelium for a comparison to see if anything obvious pops up...maybe this is something we can try in the new year!

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27 Nov 12:47

So much goddamn turkey! Anyone else's family attempt complicated routes to get mushrooms from you, prior to the holidays? Like secret drop spots, attempts at mailing fresh mushrooms, maybe i can just fruit a block at home in time!? Fun stuff on repeat every year! 

20 Nov 08:55

...aside from the concrete walls and old wooden agaricus racks they spray down between flushes...the reason why this works is ambient humidity cannot carry blotch like direct humidification systems. So the goal of a smaller and newer fruiting systems is to have fast and less frequent air exchanges to allow for as much down time as possible in the fruiting rooms. Another way to look at fruting kings is how Far West Fungi goes about it. They wait for pins to form inside of a clear and fully colonized bag and then they strip the entire bag (commonly considered counterintuitive to fruiting kings) but alas, their kings are always happy and healthy and free of blotch because humidification water cannot be trapped in the top fruiting plenum and turn into a blotch colony. The trick to this is timing. We will delve into a deep dive for you shortly!!!

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20 Nov 08:51

Hell yea ,we can jam into a King Episode for sure. Somethings to keep in mind for kings, blotch will be your constant enemy as you expand and fruiting rooms become more dense, but there are some solutions. I see a lot of folks running constant air exchange these days in fruiting rooms and failing with kings, this is a direct effect of too much air exchange with humidity either struggling to keep up or remaining at a constant. Kings will always love cooler temps (50-60F) but in terms of a fruiting room design your goal must be to keep a co2 meter modulating negative pressure protocol. This means a powerful enough fan to bring the co2 in your room down quickly enough to allow for plenty of calm air in your fruiting room. This ebb and flow will most certainly benefit your kings. For example, the most fool proof kings I've encountered were at KSS down in Kennet Square. They have extremely over air handlers and conditioners that do not pop on much and absolutely no humidification systems...

13 Nov 12:56

So we preform monthly and weekly tests for different filters, weekly for flowhoods and monthly for overpressure filters: Pressure tests and plate tests. Id say if a fair amount of dust collected on the filter back or face its worth replacing, I know the HEPA's we use can be relaced but some make it extremely difficult to do so. Usually you would pull the front grill off and remove the filter through the face and replace and re gasket the seals. If its not too dusty try and run it and test cfm as well as plate tests to determine viability!

13 Nov 12:52

Have you messed around with pho-tray tech? basically laying an XLS-A bag on its side to simulate a tray?

13 Nov 12:43

Sick!!!