Thank Tony! In the incubation space our temps are 66F and humidity does fluctuate but that doesn't make much of a difference for varieties fruited inside of an XLS-A patched bag because of the air exchange restriction. Gas exchange is one thing but you cannot get forced air out of an A patch bag that's sealed well. Lighting is something that really helps with robust invitro yeilds. Specifically in the case of Reishi, Lions Mane and Maitake...If you can see well so can they, I say. Our incubation spaces in general run in the range of 1500-2500PPM wise, this is important to consider for many reasons but mostly due to temp increasing with PPMs and autofruiting increasing with lower PPMs.

It depends on a number of factors such as the species, the desire for clear, amber or sediment free LC, will it be aerated or magnetically stirred, or perhaps both, will the aeration be constant and rolling or atomized through an airstone, or will it be a slow blub blub? We have been sticking to a pretty simple recipe:

10g Light Malt Extract

10g Dextrose

1g Soy Peptone

per 1000ml of distilled h20

On agar its a much simpler equation because the mycelium isn't pulling nutrients from deep down in the dense agar, its only growing on the top most surface. In the matrix of an aqueous solution we are finding that sediment and and a 2-3% sugar content is somewhere close to ideal for a number of common species. 

Something that still amazes me is that mycelium/mushrooms are incredible bioreactors. A diverse diet will keep basidiomycetes entertained/challenged and robust. So change up their diet and bring them to the brink of cell death and they will surprise you with adaptability and yields.

We are still waiting on their return and release so we can get them test them and find out what's up! But we are stoked to share it with everyone!

looking slick mang!!!

That's why we make em! There is something to getting the combo down in the processing that makes for insanely fast h20 uptake!  

So basic boiler water treatment can consist of a softener, carbon scrubber and chemical injection. but softening and carbon scrubbing is usually adequate to extend the life of an element or fire tubes. Sioux has an inline water treatment system that's essentially a treatment puck that slowly dissolves in X number of gallons of inline water feed. That particular treatment cup and puck isn't meant specifically for Sioux and can benefit any boiler system. We can make an episode on this in due time to expand on steam generation if folks are interested. I usually point folks in two directions for extracts. If you like Soxhlet extracts go with Fruiting Bodies out of MA. Roger is the nicest dude. For UltraSonic methods go with MycoLove out of Colorado...amazing folks and interesting intersections of methods of extraction. These two companies are serious as hell about their extract game and come highly recommended by many others than just me.

Hell ya so stoked the content is helping you and getting you stoked...we can definitely do a short video on agar cubes submerged in h2O in slants, that's a cool one. The real trick to that is not over tightening the cap of the slant and wrapping parafilm around it. KOM is certainly fast as a mutha! We will get on making that flick as well! Thank you for the suggestions

Dude, the traffic in Maine was something else! Riding two wheeled deathtraps was not advisable but we did it anyways!

I do love books, perhaps in the future this will be a thing! One thing our new website will contain is a lot more of this in writing for quick references, but the organization of all the info is a tough one thats for sure!

Yea the sound is something we are working on! Thanks for yall patience as we gear up with better equipment and settings for these tender little buggers we call mics.