So often just pairwise on plates but I have tried some pf-tek but without some downstream sequencing there will be no way to confirm what kind of crosses those are.
I have been working with fairly small block batches so I actually boil water before adding it to the bag. This dramatically reduces run time for my small inefficient steamer. I usually wait for the inner core of blocks to get to 99C and leave for 1 hour, then I switch everything off and leave it to continue pasteurising overnight. When I open up the steamer in the morning the blocks are still usually at ~75-85C.
Commented on Crystorage:I'm in Costa Rica, and sin...
08 May 17:58
How stable is the power? Freeze thaw cycles are usually more damaging. You would be better off just doing submerged agar wedges those are generally stable for a couple of years if you keep them in a cool dark cupboard