Oliver Windram

So often just pairwise on plates but I have tried some pf-tek but without some downstream sequencing there will be no way to confirm what kind of crosses those are.

I have been working with fairly small block batches so I actually boil water before adding it to the bag. This dramatically reduces run time for my small inefficient steamer. I usually wait for the inner core of blocks to get to 99C and leave for 1 hour, then I switch everything off and leave it to continue pasteurising overnight. When I open up the steamer in the morning the blocks are still usually at ~75-85C.

26 Jan 21:25

Probes till holding up been through a few cycles but I have started placing them in pp baggies to keep the corrosion down.

Posted

07 Jan 11:44

Hi All, newly joined. Zimbabwean born, UK Phd plant scientist specialising in plant fungal interactions now living in Katy, Texas. Been doing road trips throughout the US cloning wild cultivars and have recently started crossing commercial lines with some of the heat tolerant oysters I have found here in Texas to see if I can improve high temp tolerance during incubation and fruiting. Would be interested to know if anyone is working on using ITS or next-gen sequencing to try and characterise strains. My background expertise in plant science is actually using transcriptomics and phenomics to relate genetics to plant physiology. A lot of this is potentially transferable to mushroom cultivation and breeding so I was just curious if anyone is working on anything similar. Anyway still learning a lot about the practicality of growing at scale which is why I joined. Thought some of you might be interested in my janky Madgetec alternative. I have been trialling these meat thermometers in my substrate pasteurisation runs. Quite useful each probe has two temp sensors. If you place one at the centre of the bag and the other close to the surface you can track temperature transference during your run. Also as they are wireless I can track temp in real time. Not sure how long they will last but I got them cheap, second hand, so thought it was worth messing around with them.

3

07 Jan 11:19

Bit like hydrofoggers right you have to keep cleaning them. Any anti-microbial unless it is prokaryote specific will probably negatively affect your mushrooms. Even if it is a specific bactericide might I would prefer not to use it if possible.