Gregor You just have to be sure all the units of measurement are the same when using m1v1=m2v2 and define what variable you are actually searching for. Also, that equation mainly deals with Molarity as the concentration factor and percentages don't always translate nicely to the actual chemical concentration based on moles/per liter of solution. Another way to solve for it would be to calculate out the grams of water in 1.5 quarts. Then with total grams X by .03 to get 3 percent of the weight of that solution. This 3 percent in grams is how much hydrogen peroxide exists in the solution and will eventually exist in the 10 gallons of water. So then you can just divide that grams of hydrogen peroxide by 10 gallons of water in grams and this will give you the percentage of hydrogen peroxide in that total 10 gallons of water. Which comes out to .134 % which would be your true final percentage if calculating by weights instead of percentages. I can post the math if you wanna visual it.

You would use that equation to find out the concentration of your final solution, since we know the volume and concentration of our first mixture. 1.5 qts= 0.375 gal. and we know our final volume (10 gallons), so we want to discover what that final concentration in the 10 gallons is. So the setup is (.03)(.375)=(x)(10). which comes out to a final concentration of .001125. times by 100 and .1125 % is our final answer.

Peter wettlaufer I do apologize we have changed the way we store our Pink Oysters and are trying out new methods. I have just been removed from spawn for a little bit now and aren't always updated lol. So we just preserve our pink oyster with slants. The slant seems to stay stable for ~6 months before noticing problems. The petri plates will try to auto fruit after about 3 months and push through the parafilm, so we deem it not good for G1 production. Cryo was hit or miss sometimes, kind of like golds where they don't always survive the intensity of cryo so we moved away. We are looking to try an agar piece submerged in DI water and a low salt concentration for longer term (1 year) storage. Again, sorry for the mislead on my first answer but this is our current protocol.

very interesting!! Thank you for sharing so much data it was a great read and helps us get some more insight as we take on the world of liquid culture as well. It is definitely intriguing that some strains seem to stay dormant and then all of sudden attack with a full army lol. Glad it's not just seeing that. My only insight with oyster would be to try blending some grain and add that in with the LC to help with the extra nutrients and nitrogen requirements. It seems to be a looked into method for those that use LC in house, due to the fact the slurry gets too thick when placing LC in syringes.

For long term (1-2 Years) we do use cryo  and have success. In terms of regular storage, we just avoid putting any agar or G1 grain bags in refrigeration as that starts to mess with the health of the strain. For agar and G1 bags, we just then avoid using anything that starts auto-fruiting or if it passes the 3 months mark.

Sadly no leads, mainly just a lot of people getting into the cordy grow-op themsleves. You may have success reaching out to some US based tincture operations though as they are constantly popping up and looking for suppliers. Let's just say Maine enjoyed it's 4/20 🤘

We ship cultures on the retail side to Canada and have also shipped to Australia before. It seems pretty strain/country specific though, as Australia didn't allow Lions Mane. Honestly, that method should be fine but may also help to ask the delivery service if they can provide any guidance as shipping biological material is pretty common. Also in a suitcase should be perfectly fine. I have heard some good stories from my old employer of flying with a whole carry on briefcase full of anaerobic cultures ranging from all over the medical field. Also I have a feeling Erik will have some experience in this so i'll give him an ask.

Phillipe Kenny Glad I could help and hey thanks for asking questions as  a lot of these concepts and ideas are new to us as well! By asking questions you're helping us flush out these ideas as well and make sure we are still being honest to the facts or still being open to new ideas. We are all on this journey together and it's nice to bring a community along with ya!

I've really only seen that it helps with Cordycepin production and not too much other data on growth times and what not. Very cool nonetheless, how do the fruit bodies compare in size to your other bathces.

This was actually a quote I got from a medical mycology textbook. The fungi when in contact will only send the nucleus across through the septa (which most likely contains some cytoplasm.) The nuclei, are then coexisting in each cell across both colonies by DNA replication. The colonies don't transfer all their cytoplasm among each other they just share that nucleus across the colony. It's like each colony takes it upon itself to replicate and spread the new nucleus genetics across all cells rather than the colonies moving a whole bunch of nuclei over to the compatible partner. So, the nuclei aren't combing until karyogamy like you said they are just existing together in the cells. I'm sure in that bridging cell where the 2 colonies meet there has to be some cytoplasm transfer in order to move the nucleus over to the other cell and test for compatibility but that would be our only transfer.