Hello ya'll! There has been a lot of talk as of late in different forums and groups about the efficacy and practical use of old study protocols specific to chemical dedikaryotization, so i thought i would share this interesting and easy protocol for isolating two monokaryons from a single dikaryon. I'm not sure how many folks are getting as into the weeds as we are about mating and DNA analysis but this is a pretty cool primer for what is possible. Some practical reasons for needing to preform dedikaryotization is if you are trying to generate a new phenotype from a dikaryon that cannot produce mating pairs within its own spore production (i.e. Shimofuri Hiratake or PE or Enigma, among many others in the gourmet, active and medicinal world). The process of dedikaryotization allows you to pull the mon parents of literally ANY Di and from there perform crosses to continue breeding. Anyways, I hope this is interesting and insightful for ya'll and hopefully in the next couple months we will put together a full deep dive on the matter!