Hey y'all hope you enjoy our first of many sterile technique videos!! There was a minor yet important detail that wasn't mentioned. So whenever working in front of a HEPA filter be sure to never spray the isopropyl directly at the filter (due to damage). Always keep the spray bottle very slanted or even perpendicular to the filter. Also spraying with a downward angle and close to the object helps prevent the large spread of harmful isopropyl spray. Lastly, with an open culture these rules have to be followed to ensure no damage by isopropyl occurs to our live open cultures. Sorry about that and let me know if any other questions arise!